3W8H

Crystal structure of CCM3 in complex with the C-terminal regulatory domain of STK25

3W8H の概要

• エントリーDOI: 10.2210/pdb3w8h/pdb
• 関連するPDBエントリー: 3W8I
• 分子名称: Programmed cell death protein 10, Serine/threonine-protein kinase 25, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: protein binding-transferase complex, protein binding/transferase
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cytoplasm: Q9BUL8 O00506
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 34176.72

構造登録者

Xu, X.,Wang, D.C.,Ding, J. (登録日: 2013-03-13, 公開日: 2013-07-03, 最終更新日: 2023-11-08)

主引用文献

Xu, X.,Wang, X.,Zhang, Y.,Wang, D.C.,Ding, J.
Structural Basis for the Unique Heterodimeric Assembly between Cerebral Cavernous Malformation 3 and Germinal Center Kinase III.
Structure, 21:1059-1066, 2013

PubMed Abstract: Defects in cerebral cavernous malformation protein CCM3 result in cerebral cavernous malformation (CCM), a common vascular lesion of the human CNS. CCM3 functions as an adaptor protein that interacts with various signal proteins. Among these partner proteins, germinal center kinase III (GCKIII) proteins have attracted significant interest because GCKIII-CCM3 interactions play essential roles in vascular physiology. Here, we report the crystal structures of CCM3 in complex with the C-terminal regulatory domains of GCKIII (GCKIIIct) at 2.4 Å resolution. Our results reveal that GCKIIIct adopts a fold closely resembling that of the CCM3 N-terminal dimeric domain. GCKIIIct heterodimerizes with CCM3 in a manner analogous to CCM3 homodimerization. The remarkable structural rearrangement of CCM3 induced by GCKIIIct binding and the ensuing interactions within CCM3 are characterized as the structural determinants for GCKIIIct-CCM3 heterodimerization. Taken together, these findings provide a precise structural basis for GCKIIIct-CCM3 heterodimerization and the functional performance of GCKIII mediated by CCM3.

PubMed: 23665169
DOI: 10.1016/j.str.2013.04.007

実験手法

X-RAY DIFFRACTION (2.426 Å)