3W3Z

Crystal structure of Kap121p bound to RanGTP

3W3Z の概要

• エントリーDOI: 10.2210/pdb3w3z/pdb
• 関連するPDBエントリー: 3W3T 3W3U 3W3V 3W3W 3W3X 3W3Y
• 分子名称: Importin subunit beta-3, GTP-binding nuclear protein Ran, MAGNESIUM ION, ... (5 entities in total)
• 機能のキーワード: heat repeat, nuclear import, protein transport-nuclear protein complex, protein transport/nuclear protein
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast); 詳細
• 細胞内の位置: Cytoplasm (By similarity): P32337; Nucleus: P62825
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 143062.16

構造登録者

Kobayashi, J.,Matsuura, Y. (登録日: 2012-12-28, 公開日: 2013-04-10, 最終更新日: 2014-01-29)

主引用文献

Kobayashi, J.,Matsuura, Y.
Structural basis for cell-cycle-dependent nuclear import mediated by the karyopherin Kap121p.
J.Mol.Biol., 425:1852-1868, 2013

PubMed Abstract: Kap121p (also known as Pse1p) is an essential karyopherin that mediates nuclear import of a plethora of cargoes including cell cycle regulators, transcription factors, and ribosomal proteins in Saccharomyces cerevisiae. It has been proposed that the spindle assembly checkpoint signaling triggers molecular rearrangements of nuclear pore complexes and thereby arrests Kap121p-mediated nuclear import at metaphase, while leaving import mediated by other karyopherins unaffected. The Kap121p-specific import inhibition is required for normal progression through mitosis. To understand the structural basis for Kap121p-mediated nuclear import and its unique regulatory mechanism during mitosis, we determined crystal structures of Kap121p in isolation and also in complex with either its import cargoes or nucleoporin Nup53p or RanGTP. Kap121p has a superhelical structure composed of 24 HEAT repeats. The structures of Kap121p-cargo complexes define a non-conventional nuclear localization signal (NLS) that has a consensus sequence of KV/IxKx1-2K/H/R. The structure of Kap121p-Nup53p complex shows that cargo and Nup53p compete for the same high-affinity binding site, explaining how Nup53p binding forces cargo release when the Kap121p-binding site of Nup53p is exposed during mitosis. Comparison of the NLS and RanGTP complexes reveals that RanGTP binding not only occludes the cargo-binding site but also forces Kap121p into a conformation that is incompatible with NLS recognition.

PubMed: 23541588
DOI: 10.1016/j.jmb.2013.02.035

実験手法

X-RAY DIFFRACTION (2.7 Å)