3W1W

Protein-drug complex

3W1W の概要

• エントリーDOI: 10.2210/pdb3w1w/pdb
• 分子名称: Ferrochelatase, mitochondrial, FE2/S2 (INORGANIC) CLUSTER, CHOLIC ACID, ... (6 entities in total)
• 機能のキーワード: heme synthesis inhibition, ferrochelatase, lyase
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Mitochondrion inner membrane; Peripheral membrane protein; Matrix side: P22830
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 89178.57

構造登録者

Ishii, R.,Gupta, V.,Yamaguchi, Y.,Handa, H.,Nureki, O. (登録日: 2012-11-21, 公開日: 2013-10-09, 最終更新日: 2023-11-08)

主引用文献

Gupta, V.,Liu, S.,Ando, H.,Ishii, R.,Tateno, S.,Kaneko, Y.,Yugami, M.,Sakamoto, S.,Yamaguchi, Y.,Nureki, O.,Handa, H.
Salicylic Acid induces mitochondrial injury by inhibiting ferrochelatase heme biosynthesis activity
Mol.Pharmacol., 84:824-833, 2013

PubMed Abstract: Salicylic acid is a classic nonsteroidal anti-inflammatory drug. Although salicylic acid also induces mitochondrial injury, the mechanism of its antimitochondrial activity is not well understood. In this study, by using a one-step affinity purification scheme with salicylic acid-immobilized beads, ferrochelatase (FECH), a homodimeric enzyme involved in heme biosynthesis in mitochondria, was identified as a new molecular target of salicylic acid. Moreover, the cocrystal structure of the FECH-salicylic acid complex was determined. Structural and biochemical studies showed that salicylic acid binds to the dimer interface of FECH in two possible orientations and inhibits its enzymatic activity. Mutational analysis confirmed that Trp301 and Leu311, hydrophobic amino acid residues located at the dimer interface, are directly involved in salicylic acid binding. On a gel filtration column, salicylic acid caused a shift in the elution profile of FECH, indicating that its conformational change is induced by salicylic acid binding. In cultured human cells, salicylic acid treatment or FECH knockdown inhibited heme synthesis, whereas salicylic acid did not exert its inhibitory effect in FECH knockdown cells. Concordantly, salicylic acid treatment or FECH knockdown inhibited heme synthesis in zebrafish embryos. Strikingly, the salicylic acid-induced effect in zebrafish was partially rescued by FECH overexpression. Taken together, these findings illustrate that FECH is responsible for salicylic acid-induced inhibition of heme synthesis, which may contribute to its antimitochondrial and anti-inflammatory function. This study establishes a novel aspect of the complex pharmacological effects of salicylic acid.

PubMed: 24043703
DOI: 10.1124/mol.113.087940

実験手法

X-RAY DIFFRACTION (2.006 Å)