Crystal structure of Saccharomyces cerevisiae Atg12-Atg5 conjugate bound to the N-terminal domain of Atg16

Summary for 3W1S

DescriptorAutophagy protein 5, Autophagy protein 16, Ubiquitin-like protein ATG12, ... (4 entities in total)
Functional Keywordsubiquitin fold, e3-like, atg3 binding, isopeptide bond between atg12 gly186 and atg5 lys149, ligase
Biological sourceSaccharomyces cerevisiae S288c (Baker's yeast)
Cellular locationCytoplasm Q12380
Preautophagosomal structure membrane; Peripheral membrane protein Q03818 P38316
Total number of polymer chains3
Total molecular weight48754.49
Noda, N.N.,Fujioka, Y.,Hanada, T.,Ohsumi, Y.,Inagaki, F. (deposition date: 2012-11-20, release date: 2012-12-26, Last modification date: 2013-06-26)
Primary citation
Noda, N.N.,Fujioka, Y.,Hanada, T.,Ohsumi, Y.,Inagaki, F.
Structure of the Atg12-Atg5 conjugate reveals a platform for stimulating Atg8-PE conjugation
Embo Rep., 14:206-211, 2013
PubMed: 23238393 (PDB entries with the same primary citation)
DOI: 10.1038/embor.2012.208
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers 0.25814 0.3% 6.1% 3.3%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation reportDownload
PDB entries from 2020-11-25