3VVV
Skich domain of NDP52
Summary for 3VVV
| Entry DOI | 10.2210/pdb3vvv/pdb |
| Related | 3VVW |
| Descriptor | Calcium-binding and coiled-coil domain-containing protein 2 (2 entities in total) |
| Functional Keywords | autophagy adaptor protein, protein transport |
| Biological source | Homo sapiens (human) |
| Cellular location | Cytoplasm, perinuclear region: Q13137 |
| Total number of polymer chains | 1 |
| Total formula weight | 14598.34 |
| Authors | Akutsu, M.,Muhlinen, N.V.,Randow, F.,Komander, D. (deposition date: 2012-07-28, release date: 2013-02-27, Last modification date: 2024-03-20) |
| Primary citation | Muhlinen, N.V.,Akutsu, M.,Ravenhill, B.J.,Foeglein, A.,Bloor, S.,Rutherford, T.J.,Freund, S.M.,Komander, D.,Randow, F. LC3C, bound selectively by a noncanonical LIR motif in NDP52, is required for antibacterial autophagy Mol.Cell, 48:329-342, 2012 Cited by PubMed Abstract: Autophagy protects cellular homeostasis by capturing cytosolic components and invading pathogens for lysosomal degradation. Autophagy receptors target cargo to autophagy by binding ATG8 on autophagosomal membranes. The expansion of the ATG8 family in higher eukaryotes suggests that specific interactions with autophagy receptors facilitate differential cargo handling. However, selective interactors of ATG8 orthologs are unknown. Here we show that the selectivity of the autophagy receptor NDP52 for LC3C is crucial for innate immunity since cells lacking either protein cannot protect their cytoplasm against Salmonella. LC3C is required for antibacterial autophagy because in its absence the remaining ATG8 orthologs do not support efficient antibacterial autophagy. Structural analysis revealed that the selectivity of NDP52 for LC3C is conferred by a noncanonical LIR, in which lack of an aromatic residue is balanced by LC3C-specific interactions. Our report illustrates that specificity in the interaction between autophagy receptors and autophagy machinery is of functional importance to execute selective autophagy. PubMed: 23022382DOI: 10.1016/j.molcel.2012.08.024 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.35 Å) |
Structure validation
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