3VRM

Structure of cytochrome P450 Vdh mutant T107A with bound vitamin D3

Summary for 3VRM

Entry DOI	10.2210/pdb3vrm/pdb
Related	3A4G 3A4H 3A4Z 3A50 3A51
Descriptor	Vitamin D(3) 25-hydroxylase, PROTOPORPHYRIN IX CONTAINING FE, (1S,3Z)-3-[(2E)-2-[(1R,3AR,7AS)-7A-METHYL-1-[(2R)-6-METHYLHEPTAN-2-YL]-2,3,3A,5,6,7-HEXAHYDRO-1H-INDEN-4-YLIDENE]ETHYLI DENE]-4-METHYLIDENE-CYCLOHEXAN-1-OL, ... (4 entities in total)
Functional Keywords	cytochrome p450, vitamin d3, monooxygenase, oxidoreductase, p450 fold, hemoprotein
Biological source	Pseudonocardia autotrophica (Amycolata autotrophica)
Cellular location	Cytoplasm (By similarity): C4B644
Total number of polymer chains	1
Total formula weight	46458.70
Authors	Nishioka, T.,Yasutake, Y.,Tamura, T. (deposition date: 2012-04-12, release date: 2013-04-17, Last modification date: 2023-11-08)
Primary citation	Yasutake, Y.,Nishioka, T.,Imoto, N.,Tamura, T. A single mutation at the ferredoxin binding site of p450 vdh enables efficient biocatalytic production of 25-hydroxyvitamin d3. Chembiochem, 14:2284-2291, 2013 Cited by PubMed: 24115473 DOI: 10.1002/cbic.201300386 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.57 Å)

Structure validation