3VOV
Crystal Structure of ROK Hexokinase from Thermus thermophilus
Summary for 3VOV
| Entry DOI | 10.2210/pdb3vov/pdb |
| Descriptor | Glucokinase, ZINC ION, GLYCEROL, ... (4 entities in total) |
| Functional Keywords | rok, hexokinase, glucokinase, sugar kinase, transferase |
| Biological source | Thermus thermophilus |
| Total number of polymer chains | 4 |
| Total formula weight | 126785.84 |
| Authors | Nakamura, T.,Kashima, Y.,Mine, S.,Oku, T.,Uegaki, K. (deposition date: 2012-02-21, release date: 2012-06-27, Last modification date: 2024-03-20) |
| Primary citation | Nakamura, T.,Kashima, Y.,Mine, S.,Oku, T.,Uegaki, K. Characterization and crystal structure of the thermophilic ROK hexokinase from Thermus thermophilus J.Biosci.Bioeng., 2012 Cited by PubMed Abstract: We characterized and determined the crystal structure of a putative glucokinase/hexokinase from Thermus thermophilus that belongs to the ROK (bacterial repressors, uncharacterized open reading frames, and sugar kinases) family. The protein possessed significant enzymatic activity against glucose and mannose, with V(max) values of 260 and 68 μmol·min(-1)·mg(-1) protein, respectively. Therefore, we concluded that the enzyme is a hexokinase. However, the hexokinase showed little catalytic capacity for galactose and fructose. Circular dichroism measurements indicated that the enzyme was structurally stable at 90°C. The crystal structure of the enzyme was determined at a resolution of 2.02 Å, with R(cryst) and R(free) values of 18.1% and 22.6%, respectively. The polypeptide structure was divided into large and small domains. The ROK consensus sequences 1 and 2 were included in the large domain. The cysteine-rich consensus sequence 2 folded into a zinc finger, and the bound zinc was confirmed by both electron density and X-ray absorption fine structure (XAFS) spectrum. The overall structure was a homotetramer that consisted of a dimer of dimers. The accessible surface area buried by the association of the dimers into the tetrameric structures was significantly higher in the T. thermophilus enzyme than in a homologous tetrameric ROK sugar kinase. PubMed: 22591843DOI: 10.1016/j.jbiosc.2012.03.018 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.02 Å) |
Structure validation
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