3VNA
Structural insights into small RNA sorting and mRNA binding by Arabidopsis Ago Mid domains
Summary for 3VNA
| Entry DOI | 10.2210/pdb3vna/pdb |
| Related | 3VNB |
| Descriptor | Protein argonaute 1, SULFATE ION (3 entities in total) |
| Functional Keywords | rossmann fold, 5'-nucleotide small rna binding, rna binding protein |
| Biological source | Arabidopsis thaliana (Mouse-ear cress) |
| Cellular location | Cytoplasm (Potential): O04379 |
| Total number of polymer chains | 1 |
| Total formula weight | 17891.60 |
| Authors | Yuan, Y.A.,Zha, X. (deposition date: 2012-01-11, release date: 2012-10-10, Last modification date: 2024-03-20) |
| Primary citation | Zha, X.,Xia, Q.,Yuan, Y.A. Structural insights into small RNA sorting and mRNA target binding by Arabidopsis Argonaute Mid domains Febs Lett., 586:3200-3207, 2012 Cited by PubMed Abstract: The RISC-associated Argonaute (Ago) proteins play the catalytic role for RISC-mediated gene regulation by selecting small RNAs and subsequent targeting and cleavage of complementary mRNAs. Ago Mid domains are proposed to play essential roles in small RNA sorting. Here, we report the crystal structures of Arabidopsis Ago1 Mid domain and its chimera mutant with part of Ago1 replaced by Ago4. The structures demonstrate that a single amino insertion in the nucleotide specificity loop of AtAgo1 will change the nucleotide binding preference of AtAgo1 from "5'-U" to "5'-A". Moreover, we identify a long positively charged groove located along the "5'-end-nucleotide specificity loop" and occupied by several sulfate ions with the distance of 9-11Å distance, indicating a putative mRNA target binding groove. PubMed: 22771475DOI: 10.1016/j.febslet.2012.06.038 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2 Å) |
Structure validation
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