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3VLC

Crystal structure of S. cerevisiae Get3 in the semi open conformation in complex with Get1 cytosolic domain at 4.5 angstrom resolution

Summary for 3VLC
Entry DOI10.2210/pdb3vlc/pdb
DescriptorATPase GET3, Golgi to ER traffic protein 1, ADENOSINE-5'-DIPHOSPHATE (3 entities in total)
Functional Keywordsatpase, membrane protein insertion, atp binding, membrane protein binding, hydrolase-transport protein complex, hydrolase/transport protein
Biological sourceSaccharomyces cerevisiae (yeast)
More
Cellular locationEndoplasmic reticulum membrane; Multi-pass membrane protein: P53192
Total number of polymer chains2
Total formula weight51134.46
Authors
Kubota, K.,Yamagata, A.,Fukai, S. (deposition date: 2011-11-30, release date: 2012-06-20, Last modification date: 2023-11-08)
Primary citationKubota, K.,Yamagata, A.,Sato, Y.,Goto-Ito, S.,Fukai, S.
Get1 stabilizes an open dimer conformation of get3 ATPase by binding two distinct interfaces
J.Mol.Biol., 422:366-375, 2012
Cited by
PubMed Abstract: Tail-anchored (TA) proteins are integral membrane proteins that possess a single transmembrane domain near their carboxy terminus. TA proteins play critical roles in many important cellular processes such as membrane trafficking, protein translocation, and apoptosis. The GET complex mediates posttranslational insertion of newly synthesized TA proteins to the endoplasmic reticulum membrane. The GET complex is composed of the homodimeric Get3 ATPase and its heterooligomeric receptor, Get1/2. During insertion, the Get3 dimer shuttles between open and closed conformational states, coupled with ATP hydrolysis and the binding/release of TA proteins. We report crystal structures of ADP-bound Get3 in complex with the cytoplasmic domain of Get1 (Get1CD) in open and semi-open conformations at 3.0- and 4.5-Å resolutions, respectively. Our structures and biochemical data suggest that Get1 uses two interfaces to stabilize the open dimer conformation of Get3. We propose that one interface is sufficient for binding of Get1 by Get3, while the second interface stabilizes the open dimer conformation of Get3.
PubMed: 22684149
DOI: 10.1016/j.jmb.2012.05.045
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (4.5 Å)
Structure validation

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