3VHX

The crystal structure of Arf6-MKLP1 (Mitotic kinesin-like protein 1) complex

3VHX の概要

• エントリーDOI: 10.2210/pdb3vhx/pdb
• 分子名称: ADP-ribosylation factor 6, Kinesin-like protein KIF23, GUANOSINE-5'-TRIPHOSPHATE, ... (6 entities in total)
• 機能のキーワード: small gtpase, gtp binding, flemming body, cytokinesis, cell cycle-signaling protein complex, cell cycle/signaling protein
• 由来する生物種: Mus musculus (mouse); 詳細
• 細胞内の位置: Golgi apparatus (By similarity): P62331; Nucleus: Q02241
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 136306.67

構造登録者

Makyio, H.,Takei, T.,Ohgi, H.,Takahashi, S.,Takatsu, H.,Ueda, T.,Kanaho, Y.,Xie, Y.,Shin, H.W.,Kamikubo, H.,Kataoka, M.,Kawasaki, M.,Kato, R.,Wakatsuki, S.,Nakayama, K. (登録日: 2011-09-12, 公開日: 2012-05-16, 最終更新日: 2023-11-08)

主引用文献

Makyio, H.,Ohgi, M.,Takei, T.,Takahashi, S.,Takatsu, H.,Katoh, Y.,Hanai, A.,Ueda, T.,Kanaho, Y.,Xie, Y.,Shin, H.W.,Kamikubo, H.,Kataoka, M.,Kawasaki, M.,Kato, R.,Wakatsuki, S.,Nakayama, K.
Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis
Embo J., 31:2590-2603, 2012

PubMed Abstract: A small GTPase, Arf6, is involved in cytokinesis by localizing to the Flemming body (the midbody). However, it remains unknown how Arf6 contributes to cytokinesis. Here, we demonstrate that Arf6 directly interacts with mitotic kinesin-like protein 1 (MKLP1), a Flemming body-localizing protein essential for cytokinesis. The crystal structure of the Arf6-MKLP1 complex reveals that MKLP1 forms a homodimer flanked by two Arf6 molecules, forming a 2:2 heterotetramer containing an extended β-sheet composed of 22 β-strands that spans the entire heterotetramer, suitable for interaction with a concave membrane surface at the cleavage furrow. We show that, during cytokinesis, Arf6 is first accumulated around the cleavage furrow and, prior to abscission, recruited onto the Flemming body via interaction with MKLP1. We also show by structure-based mutagenesis and siRNA-mediated knockdowns that the complex formation is required for completion of cytokinesis. A model based on these results suggests that the Arf6-MKLP1 complex plays a crucial role in cytokinesis by connecting the microtubule bundle and membranes at the cleavage plane.

PubMed: 22522702
DOI: 10.1038/emboj.2012.89

実験手法

X-RAY DIFFRACTION (2.81 Å)