3VFD

Human spastin AAA domain

Summary for 3VFD

• Entry DOI: 10.2210/pdb3vfd/pdb
• Descriptor: Spastin, SULFATE ION (2 entities in total)
• Functional Keywords: atpase, microtubule severing, hydrolase
• Biological source: Homo sapiens (human)
• Cellular location: Membrane ; Peripheral membrane protein . Isoform 1: Endoplasmic reticulum membrane ; Peripheral membrane protein . Isoform 3: Cytoplasm : Q9UBP0
• Total number of polymer chains: 1
• Total formula weight: 42670.72

Authors

Taylor, J.L.,White, S.R.,Lauring, B.,Kull, F.J. (deposition date: 2012-01-09, release date: 2012-05-30, Last modification date: 2023-09-13)

Primary citation

Taylor, J.L.,White, S.R.,Lauring, B.,Kull, F.J.
Crystal structure of the human spastin AAA domain.
J.Struct.Biol., 179:133-137, 2012

PubMed Abstract: Hereditary spastic paraplegia (HSP) is a motor neuron disease caused by a progressive degeneration of the motor axons of the corticospinal tract. Point mutations or exon deletions in the microtubule-severing ATPase, spastin, are responsible for approximately 40% of cases of autosomal dominant HSP. Here, we report the 3.3 Å X-ray crystal structure of a hydrolysis-deficient mutant (E442Q) of the human spastin protein AAA domain. This structure is analyzed in the context of the existing Drosophila melanogaster spastin AAA domain structure and crystal structures of other closely related proteins in order to build a more unifying framework for understanding the structural features of this group of microtubule-severing ATPases.

PubMed: 22446388
DOI: 10.1016/j.jsb.2012.03.002

Experimental method

X-RAY DIFFRACTION (3.301 Å)