3V3D

Crystal Structure of an eYFP single mutant

3V3D の概要

• エントリーDOI: 10.2210/pdb3v3d/pdb
• 関連するPDBエントリー: 1YFP 2YFP
• 分子名称: Yellow fluorescent protein, SULFATE ION (3 entities in total)
• 機能のキーワード: second harmonic imaging, fluorescent protein
• 由来する生物種: Aequorea victoria (Jellyfish)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 29257.93

構造登録者

Ngan, N.B.,Van Hecke, K.,Van Meervelt, L. (登録日: 2011-12-13, 公開日: 2012-12-26, 最終更新日: 2024-11-06)

主引用文献

De Meulenaere, E.,Nguyen Bich, N.,de Wergifosse, M.,Van Hecke, K.,Van Meervelt, L.,Vanderleyden, J.,Champagne, B.,Clays, K.
Improving the second-order nonlinear optical response of fluorescent proteins: the symmetry argument.
J.Am.Chem.Soc., 135:4061-4069, 2013

PubMed Abstract: We have successfully designed and expressed a new fluorescent protein with improved second-order nonlinear optical properties. It is the first time that a fluorescent protein has been rationally altered for this particular characteristic. On the basis of the specific noncentrosymmetry requirements for second-order nonlinear optical effects, we had hypothesized that the surprisingly low first hyperpolarizability (β) of the enhanced yellow fluorescent protein (eYFP) could be explained by centrosymmetric stacking of the chromophoric Tyr66 and the neighboring Tyr203 residue. The inversion center was removed by mutating Tyr203 into Phe203, with minor changes in the linear optical properties and even an improved fluorescence quantum yield. Structure determination by X-ray crystallography as well as linear optical characterization corroborate a correct folding and maturation. Measurement of β by means of hyper-Rayleigh scattering (HRS) as well as their analysis using quantum chemistry calculations validate our hypothesis. This observation can eventually lead to improved red fluorescent proteins for even better performance. On the basis of the specific function (second-harmonic generation), the color of its emission, and in analogy with the "fruit" names, we propose SHardonnay as the name for this Tyr203Phe mutant of eYFP.

PubMed: 23406416
DOI: 10.1021/ja400098b

実験手法

X-RAY DIFFRACTION (1.95 Å)