3V3D

Crystal Structure of an eYFP single mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	CCD
Collection date	2011-05-07
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.000
Spacegroup name	P 41 21 2
Unit cell lengths	59.858, 59.858, 165.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.290 - 1.950
R-factor	0.1868
Rwork	0.184
R-free	0.22930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1f0b
RMSD bond length	0.009
RMSD bond angle	1.323
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.290	2.060
High resolution limit [Å]	1.950	1.950
Number of reflections	21591
Completeness [%]	94.2	99.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	2M (NH4)2SO4, 0.1M Tris-HCl pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K