3UZY
Crystal structure of 5beta-reductase (AKR1D1) E120H mutant in complex with NADP+ and 5beta-dihydrotestosterone
Summary for 3UZY
Entry DOI | 10.2210/pdb3uzy/pdb |
Related | 3UZW 3UZX 3UZZ |
Descriptor | 3-oxo-5-beta-steroid 4-dehydrogenase, NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, 5-beta-DIHYDROTESTOSTERONE, ... (5 entities in total) |
Functional Keywords | aldo-keto reductase, steroid and bile acid metabolism, catalytic tetrad mutant, tim barrel, oxidoreductase |
Biological source | Homo sapiens (human) |
Cellular location | Cytoplasm: P51857 |
Total number of polymer chains | 2 |
Total formula weight | 81355.17 |
Authors | Chen, M.,Christianson, D.W.,Penning, T.M. (deposition date: 2011-12-07, release date: 2012-03-21, Last modification date: 2023-09-13) |
Primary citation | Chen, M.,Drury, J.E.,Christianson, D.W.,Penning, T.M. Conversion of Human Steroid 5beta-Reductase (AKR1D1) into 3β-Hydroxysteroid Dehydrogenase by Single Point Mutation E120H: EXAMPLE OF PERFECT ENZYME ENGINEERING. J.Biol.Chem., 287:16609-16622, 2012 Cited by PubMed: 22437839DOI: 10.1074/jbc.M111.338780 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (1.832 Å) |
Structure validation
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