3UZX

Crystal structure of 5beta-reductase (AKR1D1) E120H mutant in complex with NADP+ and epiandrosterone

Summary for 3UZX

Entry DOI	10.2210/pdb3uzx/pdb
Related	3UZW 3UZY 3UZZ
Descriptor	3-oxo-5-beta-steroid 4-dehydrogenase, NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, 5-ALPHA-ANDROSTANE-3-BETA,17BETA-DIOL, ... (6 entities in total)
Functional Keywords	aldo-keto reductase, steroid and bile acid metabolism, catalytic tetrad mutant, tim barrel, oxidoreductase
Biological source	Homo sapiens (human)
Cellular location	Cytoplasm: P51857
Total number of polymer chains	2
Total formula weight	81428.10
Authors	Chen, M.,Christianson, D.W.,Penning, T.M. (deposition date: 2011-12-07, release date: 2012-03-21, Last modification date: 2023-09-13)
Primary citation	Chen, M.,Drury, J.E.,Christianson, D.W.,Penning, T.M. Conversion of Human Steroid 5beta-Reductase (AKR1D1) into 3β-Hydroxysteroid Dehydrogenase by Single Point Mutation E120H: EXAMPLE OF PERFECT ENZYME ENGINEERING. J.Biol.Chem., 287:16609-16622, 2012 Cited by PubMed: 22437839 DOI: 10.1074/jbc.M111.338780 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.637 Å)

Structure validation