3UTN

Crystal structure of Tum1 protein from Saccharomyces cerevisiae

3UTN の概要

• エントリーDOI: 10.2210/pdb3utn/pdb
• 分子名称: Thiosulfate sulfurtransferase TUM1, DIMETHYL SULFOXIDE, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: rhodanese-like domain, sulfurtransferase, transferase
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 37520.87

構造登録者

Qiu, R.,Wang, F.,Liu, M.,Ji, C.,Gong, W. (登録日: 2011-11-26, 公開日: 2012-10-17, 最終更新日: 2023-11-08)

主引用文献

Qiu, R.,Wang, F.,Liu, M.,Lou, T.,Ji, C.
Crystal structure of the Tum1 protein from the yeast Saccharomyces cerevisiae.
Protein Pept.Lett., 19:1139-1143, 2012

PubMed Abstract: Yeast tRNA-thiouridine modification protein 1 (Tum1) plays essential role in the sulfur transfer process of Urm1 system, which in turn is involved in many important cellular processes. In the rhodanese-like domain (RLD), conserved cysteine residue is proved to be the centre of active site of sulfurtransferases and crucial for the substrate recognition. In this report, we describe the crystal structure of Tum1 protein at 1.90 A resolution which, despite consisting of two RLDs, has only one conserved cysteine residue in the C-terminal RLD. An unaccounted electron density is found near the active site, which might point to the new cofactor in the sulfur transfer mechanism.

PubMed: 22587783
DOI: 10.2174/092986612803217060

実験手法

X-RAY DIFFRACTION (1.9 Å)