3USX
Crystal structure of PGRP-S complexed with Myristic Acid at 2.28 A resolution
Summary for 3USX
| Entry DOI | 10.2210/pdb3usx/pdb |
| Related | 3C2X |
| Descriptor | Peptidoglycan recognition protein 1, MYRISTIC ACID, GLYCEROL, ... (4 entities in total) |
| Functional Keywords | immune response, secreted, antimicrobial, pgrp-s, antibiotic, peptidoglycan binding, immune system |
| Biological source | Camelus dromedarius (camel) |
| Cellular location | Secreted (By similarity): Q9GK12 |
| Total number of polymer chains | 4 |
| Total formula weight | 76366.30 |
| Authors | Yamini, S.,Sharma, P.,Sinha, M.,Kaur, P.,Sharma, S.,Singh, T.P. (deposition date: 2011-11-24, release date: 2012-01-11, Last modification date: 2024-11-13) |
| Primary citation | Sharma, P.,Yamini, S.,Dube, D.,Singh, A.,Mal, G.,Pandey, N.,Sinha, M.,Singh, A.K.,Dey, S.,Kaur, P.,Mitra, D.K.,Sharma, S.,Singh, T.P. Structural basis of the binding of fatty acids to peptidoglycan recognition protein, PGRP-S through second binding site Arch.Biochem.Biophys., 529:1-10, 2013 Cited by PubMed Abstract: Short peptidoglycan recognition protein (PGRP-S) is a member of the mammalian innate immune system. PGRP-S from Camelus dromedarius (CPGRP-S) has been shown to bind to lipopolysaccharide (LPS), lipoteichoic acid (LTA) and peptidoglycan (PGN). Its structure consists of four molecules A, B, C and D with ligand binding clefts situated at A-B and C-D contacts. It has been shown that LPS, LTA and PGN bind to CPGRP-S at C-D contact. The cleft at the A-B contact indicated features that suggested a possible binding of fatty acids including mycolic acid of Mycobacterium tuberculosis. Therefore, binding studies of CPGRP-S were carried out with fatty acids, butyric acid, lauric acid, myristic acid, stearic acid and mycolic acid which showed affinities in the range of 10(-5) to 10(-8) M. Structure determinations of the complexes of CPGRP-S with above fatty acids showed that they bound to CPGRP-S in the cleft at the A-B contact. The flow cytometric studies showed that mycolic acid induced the production of pro-inflammatory cytokines, TNF-α and IFN-γ by CD3+ T cells. The concentrations of cytokines increased considerably with increasing concentrations of mycolic acid. However, their levels decreased substantially on adding CPGRP-S. PubMed: 23149273DOI: 10.1016/j.abb.2012.11.001 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.28 Å) |
Structure validation
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