3UGX

Crystal Structure of Visual Arrestin

3UGX の概要

• エントリーDOI: 10.2210/pdb3ugx/pdb
• 関連するPDBエントリー: 3UGU
• 分子名称: S-arrestin, PENTANEDIAL, SODIUM ION, ... (7 entities in total)
• 機能のキーワード: arrestin fold, signal termination, gpcr, outer segment, signaling protein
• 由来する生物種: Bos taurus (bovine)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 188268.75

構造登録者

Batra-Safferling, R.,Granzin, J. (登録日: 2011-11-03, 公開日: 2012-02-08, 最終更新日: 2024-02-28)

主引用文献

Granzin, J.,Cousin, A.,Weirauch, M.,Schlesinger, R.,Buldt, G.,Batra-Safferling, R.
Crystal Structure of p44, a Constitutively Active Splice Variant of Visual Arrestin.
J.Mol.Biol., 416:611-618, 2012

PubMed Abstract: Visual arrestin specifically binds to photoactivated and phosphorylated rhodopsin and inactivates phototransduction. In contrast, the p44 splice variant can terminate phototransduction by binding to nonphosphorylated light-activated rhodopsin. Here we report the crystal structure of bovine p44 at a resolution of 1.85 Å. Compared to native arrestin, the p44 structure reveals significant differences in regions crucial for receptor binding, namely flexible loop V-VI and polar core regions. Additionally, electrostatic potential is remarkably positive on the N-domain and the C-domain. The p44 structure represents an active conformation that serves as a model to explain the 'constitutive activity' found in arrestin variants.

PubMed: 22306737
DOI: 10.1016/j.jmb.2012.01.028

実験手法

X-RAY DIFFRACTION (2.649 Å)