3UDG

Structure of Deinococcus radiodurans SSB bound to ssDNA

3UDG の概要

• エントリーDOI: 10.2210/pdb3udg/pdb
• 分子名称: Single-stranded DNA-binding protein, 5'-D(P*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3', THYMIDINE-5'-PHOSPHATE, ... (4 entities in total)
• 機能のキーワード: ssb, ob fold, beta-barrel, single-stranded dna-binding, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Deinococcus radiodurans; 詳細
• 細胞内の位置: Cytoplasm : Q9RY51
• タンパク質・核酸の鎖数: 9
• 化学式量合計: 163183.59

構造登録者

George, N.P.,Ngo, K.V.,Chitteni-Patu, S.,Norais, C.A.,Battista, J.R.,Cox, M.M.,Keck, J.L. (登録日: 2011-10-28, 公開日: 2012-05-16, 最終更新日: 2024-02-28)

主引用文献

George, N.P.,Ngo, K.V.,Chitteni-Pattu, S.,Norais, C.A.,Battista, J.R.,Cox, M.M.,Keck, J.L.
Structure and Cellular Dynamics of Deinococcus radiodurans Single-stranded DNA (ssDNA)-binding Protein (SSB)-DNA Complexes.
J.Biol.Chem., 287:22123-22132, 2012

PubMed Abstract: The single-stranded DNA (ssDNA)-binding protein from the radiation-resistant bacterium Deinococcus radiodurans (DrSSB) functions as a homodimer in which each monomer contains two oligonucleotide-binding (OB) domains. This arrangement is exceedingly rare among bacterial SSBs, which typically form homotetramers of single-OB domain subunits. To better understand how this unusual structure influences the DNA binding and biological functions of DrSSB in D. radiodurans radiation resistance, we have examined the structure of DrSSB in complex with ssDNA and the DNA damage-dependent cellular dynamics of DrSSB. The x-ray crystal structure of the DrSSB-ssDNA complex shows that ssDNA binds to surfaces of DrSSB that are analogous to those mapped in homotetrameric SSBs, although there are distinct contacts in DrSSB that mediate species-specific ssDNA binding. Observations by electron microscopy reveal two salt-dependent ssDNA-binding modes for DrSSB that strongly resemble those of the homotetrameric Escherichia coli SSB, further supporting a shared overall DNA binding mechanism between the two classes of bacterial SSBs. In vivo, DrSSB levels are heavily induced following exposure to ionizing radiation. This accumulation is accompanied by dramatic time-dependent DrSSB cellular dynamics in which a single nucleoid-centric focus of DrSSB is observed within 1 h of irradiation but is dispersed by 3 h after irradiation. These kinetics parallel those of D. radiodurans postirradiation genome reconstitution, suggesting that DrSSB dynamics could play important organizational roles in DNA repair.

PubMed: 22570477
DOI: 10.1074/jbc.M112.367573

実験手法

X-RAY DIFFRACTION (2.4 Å)