3U75

Structure of E230A-fructofuranosidase from Schwanniomyces occidentalis complexed with fructosylnystose

Summary for 3U75

Entry DOI	10.2210/pdb3u75/pdb
Related	3KF3 3KF5 3U14
Descriptor	Fructofuranosidase, alpha-D-glucopyranose-(1-2)-beta-D-fructofuranose-(2-1)-beta-D-fructofuranose-(2-1)-beta-D-fructofuranose-(2-1)-beta-D-fructofuranose, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (4 entities in total)
Functional Keywords	glycosidase gh32, beta-propeller, hydrolase, carbohydrate/sugar binding, glycosylations
Biological source	Schwanniomyces occidentalis (Yeast)
Total number of polymer chains	4
Total formula weight	249162.80
Authors	Sainz-Polo, M.A.,Sanz-Aparicio, J. (deposition date: 2011-10-13, release date: 2012-04-25, Last modification date: 2023-11-01)
Primary citation	Sainz-Polo, M.A.,Gonzalez-Perez, D.,Gonzalez, B.,Plou, F.J.,Fernandez-Lobato, M.,Sanz-Aparicio, J. Structural and kinetic insights reveal that the amino acid pair GLN228/ASN254 modulates the transfructosylating specificity of Schwanniomyces occidentalis beta-fructofuranosidase, an enzyme that produces prebiotics. J.Biol.Chem., 287:19674-19686, 2012 Cited by PubMed: 22511773 DOI: 10.1074/jbc.M112.355503 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.68 Å)

Structure validation