3U14

Structure of D50A-fructofuranosidase from Schwanniomyces occidentalis complexed with inulin

Summary for 3U14

• Entry DOI: 10.2210/pdb3u14/pdb
• Related: 3KF3 3KF5
• Descriptor: Fructofuranosidase, beta-D-fructofuranose-(2-1)-beta-D-fructofuranose-(2-1)-beta-D-fructofuranose-(2-1)-beta-D-fructofuranose-(2-1)-beta-D-fructofuranose-(2-1)-beta-D-fructofuranose, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (4 entities in total)
• Functional Keywords: glycosidase gh32, beta-propeller, hydrolase, carbohydrate/sugar binding, glycosylations
• Biological source: Schwanniomyces occidentalis (Yeast)
• Total number of polymer chains: 2
• Total formula weight: 125929.17

Authors

Sainz-Polo, M.A.,Sanz-Aparicio, J. (deposition date: 2011-09-29, release date: 2012-04-25, Last modification date: 2023-11-01)

• Primary citation: Sainz-Polo, M.A.,Gonzalez-Perez, D.,Gonzalez, B.,Plou, F.J.,Fernandez-Lobato, M.,Sanz-Aparicio, J.; Structural and kinetic insights reveal that the amino acid pair GLN228/ASN254 modulates the transfructosylating specificity of Schwanniomyces occidentalis beta-fructofuranosidase, an enzyme that produces prebiotics.; J.Biol.Chem., 287:19674-19686, 2012; PubMed: 22511773; DOI: 10.1074/jbc.M112.355503

Experimental method

X-RAY DIFFRACTION (2.24 Å)