3U75
Structure of E230A-fructofuranosidase from Schwanniomyces occidentalis complexed with fructosylnystose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2010-10-25 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 1 |
Unit cell lengths | 65.207, 92.663, 117.753 |
Unit cell angles | 89.91, 82.96, 85.82 |
Refinement procedure
Resolution | 50.000 - 2.680 |
R-factor | 0.24483 |
Rwork | 0.241 |
R-free | 0.30798 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3kf3 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.820 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 116.860 |
High resolution limit [Å] | 2.680 |
Rmerge | 0.220 |
Number of reflections | 74146 |
Completeness [%] | 96.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIQUID DIFFUSION | 6 | 293 | 16% PEG 6K, 3% MPD, 0.2M MgCl2, 0.1M Mes pH 6, LIQUID DIFFUSION, temperature 293K |