3U6Y

Crystal structure of ALBA2-DNA complex

Summary for 3U6Y

• Entry DOI: 10.2210/pdb3u6y/pdb
• Related: 2h9u
• Descriptor: DNA/RNA-binding protein Alba 2, DNA (5'-D(*CP*CP*GP*GP*C)-3'), DNA (5'-D(*GP*GP*CP*CP*G)-3'), ... (5 entities in total)
• Functional Keywords: alba 2, archaea, dna-binding protein, structural genomics, nppsfa, national project on protein structural and functional analyses, riken structural genomics/proteomics initiative, rsgi, dna binding protein-dna complex, dna binding protein/dna
• Biological source: Aeropyrum pernix; More
• Total number of polymer chains: 4
• Total formula weight: 25891.39

Authors

Kumarevel, T.,Tanaka, T.,RIKEN Structural Genomics/Proteomics Initiative (RSGI) (deposition date: 2011-10-13, release date: 2012-02-22, Last modification date: 2024-11-13)

Primary citation

Tanaka, T.,Padavattan, S.,Kumarevel, T.
Crystal structure of archaeal chromatin protein Alba2-double-stranded DNA complex from Aeropyrum pernix K1.
J.Biol.Chem., 287:10394-10402, 2012

PubMed Abstract: All thermophilic and hyperthermophilic archaea encode homologs of dimeric Alba (Sac10b) proteins that bind cooperatively at high density to DNA. Here, we report the 2.0 Å resolution crystal structure of an Alba2 (Ape10b2)-dsDNA complex from Aeropyrum pernix K1. A rectangular tube-like structure encompassing duplex DNA reveals the positively charged residues in the monomer-monomer interface of each dimer packing on either side of the bound dsDNA in successive minor grooves. The extended hairpin loop connecting strands β3 and β4 undergoes significant conformational changes upon DNA binding to accommodate the other Alba2 dimer during oligomerization. Mutational analysis of key interacting residues confirmed the specificity of Alba2-dsDNA interactions.

PubMed: 22334696
DOI: 10.1074/jbc.M112.343210

Experimental method

X-RAY DIFFRACTION (2 Å)