3U39

Crystal Structure of the apo Bacillus Stearothermophilus phosphofructokinase

Summary for 3U39

• Entry DOI: 10.2210/pdb3u39/pdb
• Descriptor: 6-phosphofructokinase, CALCIUM ION (3 entities in total)
• Functional Keywords: pfk, transferase
• Biological source: Geobacillus stearothermophilus
• Cellular location: Cytoplasm : P00512
• Total number of polymer chains: 4
• Total formula weight: 136871.80

Authors

Mosser, R.,Reddy, M.C.M.,Bruning, J.B.,Sacchettini, J.C.,Reinhart, G.D. (deposition date: 2011-10-05, release date: 2012-03-28, Last modification date: 2023-09-13)

Primary citation

Mosser, R.,Reddy, M.C.,Bruning, J.B.,Sacchettini, J.C.,Reinhart, G.D.
Structure of the apo form of Bacillus stearothermophilus phosphofructokinase.
Biochemistry, 51:769-775, 2012

PubMed Abstract: The crystal structure of the unliganded form of Bacillus stearothermophilus phosphofructokinase (BsPFK) was determined using molecular replacement to 2.8 Å resolution (Protein Data Bank entry 3U39 ). The apo BsPFK structure serves as the basis for the interpretation of any structural changes seen in the binary or ternary complexes. When the apo BsPFK structure is compared with the previously published liganded structures of BsPFK, the structural impact that the binding of the ligands produces is revealed. This comparison shows that the apo form of BsPFK resembles the substrate-bound form of BsPFK, a finding that differs from previous predictions.

PubMed: 22212099
DOI: 10.1021/bi201548p

Experimental method

X-RAY DIFFRACTION (2.7921 Å)