3TYD
Dihydropteroate Synthase in complex with PPi and DHP+
Summary for 3TYD
| Entry DOI | 10.2210/pdb3tyd/pdb |
| Related | 1TWS |
| Descriptor | Dihydropteroate synthase, PYROPHOSPHATE 2-, 2-amino-6-methylidene-6,7-dihydropteridin-4(3H)-one, ... (5 entities in total) |
| Functional Keywords | anthracis, folate biosynthesis, dihydropteroate, pterine, tim barrel, transferase |
| Biological source | Bacillus anthracis (anthrax,anthrax bacterium) |
| Total number of polymer chains | 2 |
| Total formula weight | 67626.47 |
| Authors | Yun, M.-K.,White, S.W. (deposition date: 2011-09-24, release date: 2012-03-14, Last modification date: 2023-09-13) |
| Primary citation | Yun, M.K.,Wu, Y.,Li, Z.,Zhao, Y.,Waddell, M.B.,Ferreira, A.M.,Lee, R.E.,Bashford, D.,White, S.W. Catalysis and sulfa drug resistance in dihydropteroate synthase. Science, 335:1110-1114, 2012 Cited by PubMed Abstract: The sulfonamide antibiotics inhibit dihydropteroate synthase (DHPS), a key enzyme in the folate pathway of bacteria and primitive eukaryotes. However, resistance mutations have severely compromised the usefulness of these drugs. We report structural, computational, and mutagenesis studies on the catalytic and resistance mechanisms of DHPS. By performing the enzyme-catalyzed reaction in crystalline DHPS, we have structurally characterized key intermediates along the reaction pathway. Results support an S(N)1 reaction mechanism via formation of a novel cationic pterin intermediate. We also show that two conserved loops generate a substructure during catalysis that creates a specific binding pocket for p-aminobenzoic acid, one of the two DHPS substrates. This substructure, together with the pterin-binding pocket, explains the roles of the conserved active-site residues and reveals how sulfonamide resistance arises. PubMed: 22383850DOI: 10.1126/science.1214641 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.5 Å) |
Structure validation
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