3TW4

Crystal Structure of Human Septin 7 GTPase Domain

3TW4 の概要

• エントリーDOI: 10.2210/pdb3tw4/pdb
• 分子名称: Septin-7, GUANOSINE-5'-DIPHOSPHATE (2 entities in total)
• 機能のキーワード: gtpase, septins, cell cycle
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm : Q16181
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 63163.77

構造登録者

Serrao, V.H.B.,Alessandro, F.,Pereira, H.M.,Thiemann, O.T.,Garratt, R.C. (登録日: 2011-09-21, 公開日: 2011-11-23, 最終更新日: 2023-09-13)

主引用文献

Serrao, V.H.,Alessandro, F.,Caldas, V.E.,Marcal, R.L.,D'Muniz Pereira, H.,Thiemann, O.H.,Garratt, R.C.
Promiscuous interactions of human septins: The GTP binding domain of SEPT7 forms filaments within the crystal.
Febs Lett., 585:3868-3873, 2011

PubMed Abstract: We describe the purification, crystallization and structure for the GTP-binding domain of human septin 7 (SEPT7G). We show that it forms filaments within the crystal lattice which employ both the G and NC interfaces, similar to those seen in the hetero-filament of SEPT2/6/7. The NC interface is considered promiscuous as it is absent from the hetero-filament. Such promiscuity could provide the potential for permuting monomers along a filament in order to generate diversity in hetero-polymers. On the other hand, our results suggest that the G and NC interfaces may be necessary but insufficient for determining correct hetero-filament assembly.

PubMed: 22064074
DOI: 10.1016/j.febslet.2011.10.043

実験手法

X-RAY DIFFRACTION (3.35 Å)