3TU8

Crystal Structure of the Burkholderia Lethal Factor 1 (BLF1)

Summary for 3TU8

• Entry DOI: 10.2210/pdb3tu8/pdb
• Related: 3TUA
• Descriptor: Burkholderia Lethal Factor 1 (BLF1), BROMIDE ION (3 entities in total)
• Functional Keywords: toxin, unknown function
• Biological source: Burkholderia pseudomallei
• Total number of polymer chains: 1
• Total formula weight: 23324.68

Authors

Cruz, A.,Hautbergue, G.M.,Artymiuk, P.J.,Baker, P.J.,Chang, C.T.,Mahadi, N.M.,Mobbs, G.W.,Mohamed, R.,Nathan, S.,Partridge, L.J.,Raih, M.F.,Ruzheinikov, S.N.,Sedelnikova, S.E.,Wilson, S.A.,Rice, D.W. (deposition date: 2011-09-16, release date: 2011-11-30, Last modification date: 2024-10-16)

Primary citation

Cruz-Migoni, A.,Hautbergue, G.M.,Artymiuk, P.J.,Baker, P.J.,Bokori-Brown, M.,Chang, C.T.,Dickman, M.J.,Essex-Lopresti, A.,Harding, S.V.,Mahadi, N.M.,Marshall, L.E.,Mobbs, G.W.,Mohamed, R.,Nathan, S.,Ngugi, S.A.,Ong, C.,Ooi, W.F.,Partridge, L.J.,Phillips, H.L.,Raih, M.F.,Ruzheinikov, S.,Sarkar-Tyson, M.,Sedelnikova, S.E.,Smither, S.J.,Tan, P.,Titball, R.W.,Wilson, S.A.,Rice, D.W.
A Burkholderia pseudomallei toxin inhibits helicase activity of translation factor eIF4A.
Science, 334:821-824, 2011

PubMed Abstract: The structure of BPSL1549, a protein of unknown function from Burkholderia pseudomallei, reveals a similarity to Escherichia coli cytotoxic necrotizing factor 1. We found that BPSL1549 acted as a potent cytotoxin against eukaryotic cells and was lethal when administered to mice. Expression levels of bpsl1549 correlate with conditions expected to promote or suppress pathogenicity. BPSL1549 promotes deamidation of glutamine-339 of the translation initiation factor eIF4A, abolishing its helicase activity and inhibiting translation. We propose to name BPSL1549 Burkholderia lethal factor 1.

PubMed: 22076380
DOI: 10.1126/science.1211915

Experimental method

X-RAY DIFFRACTION (1.04 Å)