3TQ6

Crystal structure of human mitochondrial transcription factor A, TFAM or mtTFA, bound to the light strand promoter LSP

3TQ6 の概要

• エントリーDOI: 10.2210/pdb3tq6/pdb
• 分子名称: Transcription factor A, mitochondrial, DNA (5'-D(*TP*AP*AP*CP*AP*GP*TP*CP*AP*CP*CP*CP*CP*CP*CP*AP*AP*CP*(BRU)P*AP*AP*C)-3'), DNA (5'-D(*GP*TP*TP*AP*GP*TP*TP*GP*GP*GP*GP*GP*GP*TP*GP*AP*CP*TP*GP*TP*TP*A)-3'), ... (7 entities in total)
• 機能のキーワード: transcription, transcription regulation, mitochondrion, dna-binding, hmgb-ubf_hmg-box, minor groove, tandem hmg boxes, transcription-dna complex, mitochondrial nucleoid, light-strand mitochondrial promoter, lsp, transcription/dna
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Mitochondrion: Q00059
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 78993.82

構造登録者

Rubio-Cosials, A.,Sydow, J.F.,Jimenez-Menendez, N.,Fernandez-Millan, P.,Montoya, J.,Jacobs, H.T.,Coll, M.,Bernado, P.,Sola, M. (登録日: 2011-09-09, 公開日: 2011-11-02, 最終更新日: 2024-02-28)

主引用文献

Rubio-Cosials, A.,Sidow, J.F.,Jimenez-Menendez, N.,Fernandez-Millan, P.,Montoya, J.,Jacobs, H.T.,Coll, M.,Bernado, P.,Sola, M.
Human mitochondrial transcription factor A induces a U-turn structure in the light strand promoter.
Nat.Struct.Mol.Biol., 18:1281-1289, 2011

PubMed Abstract: Human mitochondrial transcription factor A, TFAM, is essential for mitochondrial DNA packaging and maintenance and also has a crucial role in transcription. Crystallographic analysis of TFAM in complex with an oligonucleotide containing the mitochondrial light strand promoter (LSP) revealed two high-mobility group (HMG) protein domains that, through different DNA recognition properties, intercalate residues at two inverted DNA motifs. This induced an overall DNA bend of ~180°, stabilized by the interdomain linker. This U-turn allows the TFAM C-terminal tail, which recruits the transcription machinery, to approach the initiation site, despite contacting a distant DNA sequence. We also ascertained that structured protein regions contacting DNA in the crystal were highly flexible in solution in the absence of DNA. Our data suggest that TFAM bends LSP to create an optimal DNA arrangement for transcriptional initiation while facilitating DNA compaction elsewhere in the genome.

PubMed: 22037172
DOI: 10.1038/nsmb.2160

実験手法

X-RAY DIFFRACTION (2.45 Å)