3TOC

Crystal structure of Streptococcus pyogenes Csn2

3TOC の概要

• エントリーDOI: 10.2210/pdb3toc/pdb
• 分子名称: Putative uncharacterized protein, CALCIUM ION, 1,2-ETHANEDIOL, ... (4 entities in total)
• 機能のキーワード: dna binding protein
• 由来する生物種: Streptococcus pyogenes
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 52715.45

構造登録者

Bae, E.,Jung, D.K.,Koo, Y. (登録日: 2011-09-05, 公開日: 2012-05-30, 最終更新日: 2024-10-30)

主引用文献

Koo, Y.,Jung, D.K.,Bae, E.
Crystal Structure of Streptococcus pyogenes Csn2 Reveals Calcium-Dependent Conformational Changes in Its Tertiary and Quaternary Structure
Plos One, 7:e33401-e33401, 2012

PubMed Abstract: Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins constitute a microbial immune system against invading genetic elements, such as plasmids and phages. Csn2 is an Nmeni subtype-specific Cas protein, and was suggested to function in the adaptation process, during which parts of foreign nucleic acids are integrated into the host microbial genome to enable immunity against future invasion. Here, we report a 2.2 Å crystal structure of Streptococcus pyogenes Csn2. The structure revealed previously unseen calcium-dependent conformational changes in its tertiary and quaternary structure. This supports the proposed double-stranded DNA-binding function of S. pyogenes Csn2.

PubMed: 22479393
DOI: 10.1371/journal.pone.0033401

実験手法

X-RAY DIFFRACTION (2.201 Å)