3TJZ

Crystal Structure of Arf1 Bound to the gamma/zeta-COP Core Complex

3TJZ の概要

• エントリーDOI: 10.2210/pdb3tjz/pdb
• 分子名称: ADP-ribosylation factor 1, Coatomer subunit gamma, Coatomer subunit zeta-1, ... (5 entities in total)
• 機能のキーワード: protein trafficking, golgi membrane, protein transport-protein binding complex, protein transport/protein binding
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast); 詳細
• 細胞内の位置: Golgi apparatus: P11076; Cytoplasm: P53620; Cytoplasm (By similarity): P35604
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 152134.40

構造登録者

Goldberg, J.,Yu, X.,Breitman, M. (登録日: 2011-08-25, 公開日: 2012-02-22, 最終更新日: 2024-02-28)

主引用文献

Yu, X.,Breitman, M.,Goldberg, J.
A structure-based mechanism for arf1-dependent recruitment of coatomer to membranes.
Cell(Cambridge,Mass.), 148:530-542, 2012

PubMed Abstract: Budding of COPI-coated vesicles from Golgi membranes requires an Arf family G protein and the coatomer complex recruited from cytosol. Arf is also required with coatomer-related clathrin adaptor complexes to bud vesicles from the trans-Golgi network and endosomal compartments. To understand the structural basis for Arf-dependent recruitment of a vesicular coat to the membrane, we determined the structure of Arf1 bound to the γζ-COP subcomplex of coatomer. Structure-guided biochemical analysis reveals that a second Arf1-GTP molecule binds to βδ-COP at a site common to the γ- and β-COP subunits. The Arf1-binding sites on coatomer are spatially related to PtdIns4,5P(2)-binding sites on the endocytic AP2 complex, providing evidence that the orientation of membrane binding is general for this class of vesicular coat proteins. A bivalent GTP-dependent binding mode has implications for the dynamics of coatomer interaction with the Golgi and for the selection of cargo molecules.

PubMed: 22304919
DOI: 10.1016/j.cell.2012.01.015

実験手法

X-RAY DIFFRACTION (2.9 Å)