Summary for 3THK
| Entry DOI | 10.2210/pdb3thk/pdb |
| Related | 1PWT 1SHG |
| NMR Information | BMRB: 7033 |
| Descriptor | Spectrin alpha chain, brain, Proline-rich peptide, BETA-MERCAPTOETHANOL, ... (5 entities in total) |
| Functional Keywords | sh3 domain, chimera, structural protein |
| Biological source | Rattus norvegicus (brown rat,rat,rats) More |
| Cellular location | Cytoplasm, cytoskeleton (By similarity): P16086 |
| Total number of polymer chains | 4 |
| Total formula weight | 18637.17 |
| Authors | Gabdulkhakov, A.G.,Gushchina, L.V.,Nikulin, A.D.,Nikonov, S.V.,Filimonov, V.V. (deposition date: 2011-08-19, release date: 2011-11-23, Last modification date: 2023-09-13) |
| Primary citation | Gushchina, L.V.,Gabdulkhakov, A.G.,Nikonov, S.V.,Filimonov, V.V. High-Resolution Crystal Structure of Spectrin SH3 Domain Fused with a Proline-Rich Peptide. J.Biomol.Struct.Dyn., 29:485-495, 2011 Cited by PubMed Abstract: A new chimeric protein, named WT-CIIA, was designed by connecting the proline-rich decapeptide PPPVPPYSAG to the C-terminus of the alpha-spectrin SH3 domain through a natural twelve-residue linker to obtain a single-chain model that would imitate intramolecular SH3-ligand interaction. The crystal structure of this fusion protein was determined at 1.7 Å resolution. The asymmetric unit of the crystal contained two SH3 globules contacting with one PPPVPPY fragment located between them. The domains are related by the two-fold non-crystallographic axis and the ligand lies in two opposite orientations with respect to the conservative binding sites of SH3 domains. PubMed: 22066535PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.7 Å) |
Structure validation
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