3TEL

LytR-CPS2A-Psr family protein with bound octaprenyl pyrophosphate lipid and manganese ion

Summary for 3TEL

• Entry DOI: 10.2210/pdb3tel/pdb
• Descriptor: Cps2A, Integral membrane regulatory protein Wzg, (2Z,6Z,10Z,14Z,18Z,22Z,26Z)-3,7,11,15,19,23,27,31-octamethyldotriaconta-2,6,10,14,18,22,26,30-octaen-1-yl trihydrogen diphosphate, MANGANESE (II) ION, ... (6 entities in total)
• Functional Keywords: cell wall techoic acid synthesis, replication, transferase
• Biological source: Streptococcus pneumoniae
• Total number of polymer chains: 1
• Total formula weight: 45111.36

Authors

Kawai, Y.,Marles-Wright, J.,Cleverley, R.M.,Emmins, R.,Ishikawa, S.,Kuwano, M.,Heinz, N.,Khai Bui, N.,Hoyland, C.N.,Ogasawara, N.,Lewis, R.J.,Vollmer, W.,Daniel, R.A.,Errington, J. (deposition date: 2011-08-15, release date: 2011-10-12, Last modification date: 2024-02-28)

Primary citation

Kawai, Y.,Marles-Wright, J.,Cleverley, R.M.,Emmins, R.,Ishikawa, S.,Kuwano, M.,Heinz, N.,Bui, N.K.,Hoyland, C.N.,Ogasawara, N.,Lewis, R.J.,Vollmer, W.,Daniel, R.A.,Errington, J.
A widespread family of bacterial cell wall assembly proteins.
Embo J., 30:4931-4941, 2011

PubMed Abstract: Teichoic acids and acidic capsular polysaccharides are major anionic cell wall polymers (APs) in many bacteria, with various critical cell functions, including maintenance of cell shape and structural integrity, charge and cation homeostasis, and multiple aspects of pathogenesis. We have identified the widespread LytR-Cps2A-Psr (LCP) protein family, of previously unknown function, as novel enzymes required for AP synthesis. Structural and biochemical analysis of several LCP proteins suggest that they carry out the final step of transferring APs from their lipid-linked precursor to cell wall peptidoglycan (PG). In Bacillus subtilis, LCP proteins are found in association with the MreB cytoskeleton, suggesting that MreB proteins coordinate the insertion of the major polymers, PG and AP, into the cell wall.

PubMed: 21964069
DOI: 10.1038/emboj.2011.358

Experimental method

X-RAY DIFFRACTION (1.8 Å)