3TEL
LytR-CPS2A-Psr family protein with bound octaprenyl pyrophosphate lipid and manganese ion
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 73.681, 73.681, 163.661 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.830 - 1.800 |
| R-factor | 0.18303 |
| Rwork | 0.181 |
| R-free | 0.21978 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.307 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.830 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 42582 | |
| Completeness [%] | 99.8 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 295 | 0.1M sodium acetate pH 4.6 9% PEG 4K, Cryprotectant solution containing 10 mM MnCl2, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
