3T95

Crystal structure of LsrB from Yersinia pestis complexed with autoinducer-2

Summary for 3T95

• Entry DOI: 10.2210/pdb3t95/pdb
• Related: 1TJY 1TM2 3EJW
• Descriptor: Autoinducer 2-binding protein lsrB, (2R,4S)-2-methyl-2,3,3,4-tetrahydroxytetrahydrofuran (3 entities in total)
• Functional Keywords: periplasmic binding protein fold, periplasmic space, signaling protein
• Biological source: Yersinia pestis
• Cellular location: Periplasm (Potential): Q74PW2
• Total number of polymer chains: 1
• Total formula weight: 36503.20

Authors

Kavanaugh, J.S.,Gakhar, L.,Horswill, A.R. (deposition date: 2011-08-02, release date: 2011-12-07, Last modification date: 2024-11-27)

Primary citation

Kavanaugh, J.S.,Gakhar, L.,Horswill, A.R.
The structure of LsrB from Yersinia pestis complexed with autoinducer-2.
Acta Crystallogr.,Sect.F, 67:1501-1505, 2011

PubMed Abstract: The crystal structure of LsrB from Yersinia pestis complexed with autoinducer-2 (AI-2; space group P2(1)2(1)2(1), unit-cell parameters a = 40.61, b = 61.03, c = 125.23 Å) has been solved by molecular replacement using the structure of LsrB from Salmonella typhimurium (PDB entry 1tjy) and refined to R = 0.180 (R(free) = 0.213) at 1.75 Å resolution. The electron density for bound AI-2 and the stereochemistry of the AI-2-binding site are consistent with bound AI-2 adopting the (2R,4S)-2-methyl-2,3,3,4-tetrahydroxytetrahydrofuran conformation, just as has been observed in the crystal structures of the Salmonella typhimurium and Sinorhizobium meliloti LsrB-AI-2 complexes.

PubMed: 22139152
DOI: 10.1107/S1744309111042953

Experimental method

X-RAY DIFFRACTION (1.75 Å)