3T12

MglA in complex with MglB in transition state

3T12 の概要

• エントリーDOI: 10.2210/pdb3t12/pdb
• 関連するPDBエントリー: 3T1O 3T1Q 3T1R 3T1S 3T1T 3T1V 3T1X
• 分子名称: Gliding protein mglA, Gliding protein MglB, GUANOSINE-5'-DIPHOSPHATE, ... (6 entities in total)
• 機能のキーワード: g-domain containing protein, bacterial polarity, motility, homodimeric gap protein, pole localisation, alpha/beta proteins, gtpase, hydrolase-signaling protein complex, hydrolase/signaling protein
• 由来する生物種: Thermus thermophilus; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 51271.19

構造登録者

Miertzschke, M.,Vetter, I.R.,Koerner, C.,Wittinghofer, A. (登録日: 2011-07-21, 公開日: 2011-08-31, 最終更新日: 2023-09-13)

主引用文献

Miertzschke, M.,Koerner, C.,Vetter, I.R.,Keilberg, D.,Hot, E.,Leonardy, S.,Sogaard-Andersen, L.,Wittinghofer, A.
Structural analysis of the Ras-like G protein MglA and its cognate GAP MglB and implications for bacterial polarity.
Embo J., 30:4185-4197, 2011

PubMed Abstract: The bacterium Myxococcus xanthus uses a G protein cycle to dynamically regulate the leading/lagging pole polarity axis. The G protein MglA is regulated by its GTPase-activating protein (GAP) MglB, thus resembling Ras family proteins. Here, we show structurally and biochemically that MglA undergoes a dramatic, GDP-GTP-dependent conformational change involving a screw-type forward movement of the central β2-strand, never observed in any other G protein. This movement and complex formation with MglB repositions the conserved residues Arg53 and Gln82 into the active site. Residues required for catalysis are thus not provided by the GAP MglB, but by MglA itself. MglB is a Roadblock/LC7 protein and functions as a dimer to stimulate GTP hydrolysis in a 2:1 complex with MglA. In vivo analyses demonstrate that hydrolysis mutants abrogate Myxococcus' ability to regulate its polarity axis changing the reversal behaviour from stochastic to oscillatory and that both MglA GTPase activity and MglB GAP catalysis are essential for maintaining a proper polarity axis.

PubMed: 21847100
DOI: 10.1038/emboj.2011.291

実験手法

X-RAY DIFFRACTION (2.2 Å)