3SO2

Chlorella dUTPase

Summary for 3SO2

• Entry DOI: 10.2210/pdb3so2/pdb
• Descriptor: Putative uncharacterized protein (2 entities in total)
• Functional Keywords: deoxyuridine triphosphatase, dutpase, chlorella, cancer marker, hydrolase
• Biological source: Chlorella variabilis
• Total number of polymer chains: 1
• Total formula weight: 15380.25

Authors

Badalucco, L.,Poudel, I.,Natarajan, c.,Yamanishi, M.,Moriyama, H. (deposition date: 2011-06-29, release date: 2012-07-04, Last modification date: 2023-09-13)

Primary citation

Badalucco, L.,Poudel, I.,Yamanishi, M.,Natarajan, C.,Moriyama, H.
Crystallization of Chlorella deoxyuridine triphosphatase.
Acta Crystallogr.,Sect.F, 67:1599-1602, 2011

PubMed Abstract: Deoxyuridine triphosphatase (dUTPase) is a ubiquitous enzyme that has been widely studied owing to its function and evolutionary significance. The gene coding for the dUTPase from the Chlorella alga was codon-optimized and synthesized. The synthetic gene was expressed in Escherichia coli and recombinant core Chlorella dUTPase (chdUTPase) was purified. Crystallization of chdUTPase was performed by the repetitive hanging-drop vapor-diffusion method at 298 K with ammonium sulfate as the precipitant. In the presence of 2'-deoxyuridine-5'-[(α,β)-imido]triphosphate and magnesium, the enzyme produced die-shaped hexagonal R3 crystals with unit-cell parameters a = b = 66.9, c = 93.6 Å, γ = 120°. X-ray diffraction data for chdUTPase were collected to 1.6 Å resolution. The crystallization of chdUTPase with manganese resulted in very fragile clusters of needles.

PubMed: 22139176
DOI: 10.1107/S1744309111038097

Experimental method

X-RAY DIFFRACTION (1.6428 Å)