3SK2

Crystal structure of phenazine resistance protein EhpR from Enterobacter agglomerans (Erwinia herbicola, Pantoea agglomerans) Eh1087 in complex with griseoluteic acid

Summary for 3SK2

• Entry DOI: 10.2210/pdb3sk2/pdb
• Descriptor: EhpR, 6-formyl-9-methoxyphenazine-1-carboxylic acid (3 entities in total)
• Functional Keywords: antibiotic resistance, griseoluteate-binding protein
• Biological source: Pantoea agglomerans (Erwinia herbicola)
• Total number of polymer chains: 2
• Total formula weight: 30126.13

Authors

Blankenfeldt, W.,Yu, S. (deposition date: 2011-06-22, release date: 2011-08-31, Last modification date: 2024-04-03)

Primary citation

Yu, S.,Vit, A.,Devenish, S.,Mahanty, H.K.,Itzen, A.,Goody, R.S.,Blankenfeldt, W.
Atomic resolution structure of EhpR: phenazine resistance in Enterobacter agglomerans Eh1087 follows principles of bleomycin / mitomycin C resistance in other bacteria.
Bmc Struct.Biol., 11:33-33, 2011

PubMed Abstract: The phenazines are redox-active secondary metabolites that a large number of bacterial strains produce and excrete into the environment. They possess antibiotic activity owing to the fact that they can reduce molecular oxygen to toxic reactive oxygen species. In order to take advantage of this activity, phenazine producers need to protect themselves against phenazine toxicity. Whereas it is believed that phenazine-producing pseudomonads possess highly active superoxide dismutases and catalases, it has recently been found that the plant-colonizing bacterium Enterobacter agglomerans expresses a small gene ehpR to render itself resistant towards D-alanyl-griseoluteic acid, the phenazine antibiotic produced by this strain.

PubMed: 21849072
DOI: 10.1186/1472-6807-11-33

Experimental method

X-RAY DIFFRACTION (1.01 Å)