3SJ4

PpcA mutant M58K

Summary for 3SJ4

• Entry DOI: 10.2210/pdb3sj4/pdb
• Related: 3SEL 3SJ0 3SJ1
• Descriptor: CYTOCHROME C7, HEME C, (3ALPHA,5BETA,12ALPHA)-3,12-DIHYDROXYCHOLAN-24-OIC ACID, ... (5 entities in total)
• Functional Keywords: three heme cytochrome c7, electron transport, three heme cyytochrome c7
• Biological source: Geobacter sulfurreducens
• Total number of polymer chains: 1
• Total formula weight: 10282.40

Authors

Pokkuluri, P.R.,Schiffer, M. (deposition date: 2011-06-20, release date: 2012-07-04, Last modification date: 2023-09-13)

Primary citation

Pokkuluri, P.R.,Yang, X.,Londer, Y.Y.,Schiffer, M.
Pitfalls in the interpretation of structural changes in mutant proteins from crystal structures.
J.Struct.Funct.Genom., 13:227-232, 2012

PubMed Abstract: PpcA is a small protein with 71 residues that contains three covalently bound hemes. The structures of single mutants at residue 58 have shown larger deviations in another part of the protein molecule than at the site of the mutation. Closer examination of the crystal packing has revealed the origin of this unexpected structural change. The site of mutation is within Van der Waals distance from another protein molecule related by a crystallographic twofold axis within the crystal. The structural changes occurred at or near the mutation site have led to a slight adjustment of the surface residues in contact. The observed deviations between the native and the mutant molecular structures are derived from the new crystal packing even though the two crystals are essentially isomorphous. Without careful consideration of the crystal lattice a non-expert looking at only the coordinates deposited in the Protein Data Bank could draw erroneous conclusion that mutation in one part of the molecule affected the structure of the protein in a distant part of the molecule.

PubMed: 23099666
DOI: 10.1007/s10969-012-9147-1

Experimental method

X-RAY DIFFRACTION (1.9 Å)