3SIT

Crystal structure of porcine CRW-8 Rotavirus VP8* in complex with aceramido-GM3

3SIT の概要

• エントリーDOI: 10.2210/pdb3sit/pdb
• 関連するPDBエントリー: 2I2S 3SIS
• 関連するBIRD辞書のPRD_ID: PRD_900025
• 分子名称: Outer capsid protein VP4, N-acetyl-alpha-neuraminic acid-(2-3)-beta-D-galactopyranose-(1-4)-alpha-D-glucopyranose, SODIUM ION, ... (5 entities in total)
• 機能のキーワード: beta sandwich, lectin, gm3, sugar binding protein, viral protein
• 由来する生物種: Porcine rotavirus (RV-A)
• 細胞内の位置: Outer capsid protein VP4: Virion. Outer capsid protein VP8*: Virion. Outer capsid protein VP5*: Virion: P0C6Y8
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 38468.46

構造登録者

Blanchard, H.,Yu, X. (登録日: 2011-06-20, 公開日: 2011-09-28, 最終更新日: 2023-09-13)

主引用文献

Yu, X.,Coulson, B.S.,Fleming, F.E.,Dyason, J.C.,von Itzstein, M.,Blanchard, H.
Novel structural insights into rotavirus recognition of ganglioside glycan receptors.
J.Mol.Biol., 413:929-939, 2011

PubMed Abstract: Rotaviruses ubiquitously infect children under the age of 5, being responsible for more than half a million diarrhoeal deaths each year worldwide. Host cell oligosaccharides containing sialic acid(s) are critical for attachment by rotaviruses. However, to date, no detailed three-dimensional atomic model showing the exact rotavirus interactions with these glycoconjugate receptors has been reported. Here, we present the first crystallographic structures of the rotavirus carbohydrate-recognizing protein VP8* in complex with ganglioside G(M3) glycans. In combination with assessment of the inhibition of rotavirus infectivity by N-acetyl and N-glycolyl forms of this ganglioside, our results reveal key details of rotavirus-ganglioside G(M3) glycan recognition. In addition, they show a direct correlation between the carbohydrate specificities exhibited by VP8* from porcine and by monkey rotaviruses and the respective infectious virus particles. These novel results also indicate the potential binding interactions of rotavirus VP8* with other sialic acid-containing gangliosides.

PubMed: 21945555
DOI: 10.1016/j.jmb.2011.09.005

実験手法

X-RAY DIFFRACTION (1.8 Å)