3RWT

Crystal structure of circular permutated Red Fluorescent Protein mKate(cp 154-153)

3RWT の概要

• エントリーDOI: 10.2210/pdb3rwt/pdb
• 関連するPDBエントリー: 3RWA
• 分子名称: Fluorescent protein FP480,Fluorescent protein FP480, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: gfp-like fluorescent protein, mkate, circular permutated, red fluorescentprotein, fluorescent protein
• 由来する生物種: Entacmaea quadricolor (Bubble-tip anemone); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 212714.45

構造登録者

Wang, Q.,Sondermann, H. (登録日: 2011-05-09, 公開日: 2011-06-15, 最終更新日: 2026-03-18)

主引用文献

Shui, B.,Wang, Q.,Lee, F.,Byrnes, L.J.,Chudakov, D.M.,Lukyanov, S.A.,Sondermann, H.,Kotlikoff, M.I.
Circular permutation of red fluorescent proteins.
Plos One, 6:e20505-e20505, 2011

PubMed Abstract: Circular permutation of fluorescent proteins provides a substrate for the design of molecular sensors. Here we describe a systematic exploration of permutation sites for mCherry and mKate using a tandem fusion template approach. Circular permutants retaining more than 60% (mCherry) and 90% (mKate) brightness of the parent molecules are reported, as well as a quantitative evaluation of the fluorescence from neighboring mutations. Truncations of circular permutants indicated essential N- and C-terminal segments and substantial flexibility in the use of these molecules. Structural evaluation of two cp-mKate variants indicated no major conformational changes from the previously reported wild-type structure, and cis conformation of the chromophores. Four cp-mKates were identified with over 80% of native fluorescence, providing important new building blocks for sensor and complementation experiments.

PubMed: 21647365
DOI: 10.1371/journal.pone.0020505

実験手法

X-RAY DIFFRACTION (3 Å)