3RU8

Structure of an HIV epitope scaffold in complex with neutralizing antibody b12 Fab

3RU8 の概要

• エントリーDOI: 10.2210/pdb3ru8/pdb
• 分子名称: Epitope Scaffold 2bodx43, Antibody b12, Heavy Chain, Antibody b12, Light Chain, ... (5 entities in total)
• 機能のキーワード: binding epitope from hiv on a heterologous protein, immune system, de novo protein
• 由来する生物種: Human immunodeficiency virus, synthetic construct, Thermomonospora fusca; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 79057.90

構造登録者

Carrico, C.T.D.,Strong, R.K. (登録日: 2011-05-04, 公開日: 2011-11-02, 最終更新日: 2024-11-06)

主引用文献

Azoitei, M.L.,Correia, B.E.,Ban, Y.E.,Carrico, C.,Kalyuzhniy, O.,Chen, L.,Schroeter, A.,Huang, P.S.,McLellan, J.S.,Kwong, P.D.,Baker, D.,Strong, R.K.,Schief, W.R.
Computation-guided backbone grafting of a discontinuous motif onto a protein scaffold.
Science, 334:373-376, 2011

PubMed Abstract: The manipulation of protein backbone structure to control interaction and function is a challenge for protein engineering. We integrated computational design with experimental selection for grafting the backbone and side chains of a two-segment HIV gp120 epitope, targeted by the cross-neutralizing antibody b12, onto an unrelated scaffold protein. The final scaffolds bound b12 with high specificity and with affinity similar to that of gp120, and crystallographic analysis of a scaffold bound to b12 revealed high structural mimicry of the gp120-b12 complex structure. The method can be generalized to design other functional proteins through backbone grafting.

PubMed: 22021856
DOI: 10.1126/science.1209368

実験手法

X-RAY DIFFRACTION (2.07 Å)