3RTR

A RING E3-substrate complex poised for ubiquitin-like protein transfer: structural insights into cullin-RING ligases

3RTR の概要

• エントリーDOI: 10.2210/pdb3rtr/pdb
• 分子名称: Cullin-1, E3 ubiquitin-protein ligase RBX1, ZINC ION (3 entities in total)
• 機能のキーワード: ubiquitin, nedd8, cullin, ubl conjugation pathway, e3 ligase, ligase
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cytoplasm: P62877
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 219919.76

構造登録者

Calabrese, M.F.,Scott, D.C.,Duda, D.M.,Grace, C.R.,Kurinov, I.,Kriwacki, R.W.,Schulman, B.A. (登録日: 2011-05-03, 公開日: 2011-07-20, 最終更新日: 2023-09-13)

主引用文献

Calabrese, M.F.,Scott, D.C.,Duda, D.M.,Grace, C.R.,Kurinov, I.,Kriwacki, R.W.,Schulman, B.A.
A RING E3-substrate complex poised for ubiquitin-like protein transfer: structural insights into cullin-RING ligases.
Nat.Struct.Mol.Biol., 18:947-949, 2011

PubMed Abstract: How RING E3 ligases mediate E2-to-substrate ubiquitin-like protein (UBL) transfer remains unknown. Here we address how the RING E3 RBX1 positions NEDD8's E2 (UBC12) and substrate (CUL1). We find that existing structures are incompatible with CUL1 NEDD8ylation and report a new conformation of RBX1 that places UBC12 adjacent to CUL1. We propose RING domain rotation as a general mechanism for UBL transfer for the largest family of E3s.

PubMed: 21765416
DOI: 10.1038/nsmb.2086

実験手法

X-RAY DIFFRACTION (3.21 Å)