3RQE

Cerebral cavernous malformation 3 (CCM3) in complex with paxillin LD1

3RQE の概要

• エントリーDOI: 10.2210/pdb3rqe/pdb
• 関連するPDBエントリー: 3AJM 3L8I 3L8J 3RQF 3RQG
• 分子名称: Programmed cell death protein 10, Paxillin LD1 peptide (2 entities in total)
• 機能のキーワード: protein-peptide complex, protein binding, fat domain, dimerization, cerebral cavernous malformation
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cytoplasm: Q9BUL8
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 101214.67

構造登録者

Li, X.,Zhang, R.,Boggon, T.J. (登録日: 2011-04-28, 公開日: 2011-06-01, 最終更新日: 2023-09-13)

主引用文献

Li, X.,Ji, W.,Zhang, R.,Folta-Stogniew, E.,Min, W.,Boggon, T.J.
Molecular Recognition of Leucine-Aspartate Repeat (LD) Motifs by the Focal Adhesion Targeting Homology Domain of Cerebral Cavernous Malformation 3 (CCM3).
J.Biol.Chem., 286:26138-26147, 2011

PubMed Abstract: Cerebral cavernous malformation (CCM) is a disease that affects between 0.1 and 0.5% of the human population, with mutations in CCM3 accounting for ~ 15% of the autosomal dominant form of the disease. We recently reported that CCM3 contains an N-terminal dimerization domain (CCM3D) and a C-terminal focal adhesion targeting (FAT) homology domain. Intermolecular protein-protein interactions of CCM3 are mediated by a highly conserved surface on the FAT homology domain and are affected by CCM3 truncations in the human disease. Here we report the crystal structures of CCM3 in complex with three different leucine-aspartate repeat (LD) motifs (LD1, LD2, and LD4) from the scaffolding protein paxillin, at 2.8, 2.7, and 2.5 Å resolution. We show that CCM3 binds LD motifs using the highly conserved hydrophobic patch 1 (HP1) and that this binding is similar to the binding of focal adhesion kinase and Pyk2 FAT domains to paxillin LD motifs. We further show by surface plasmon resonance that CCM3 binds paxillin LD motifs with affinities in the micromolar range, similar to FAK family FAT domains. Finally, we show that endogenous CCM3 and paxillin co-localize in mouse cerebral pericytes. These studies provide a molecular-level framework to investigate the protein-protein interactions of CCM3.

PubMed: 21632544
DOI: 10.1074/jbc.M110.211250

実験手法

X-RAY DIFFRACTION (2.8 Å)