3RO5

Crystal structure of influenza A virus nucleoprotein with ligand

Summary for 3RO5

• Entry DOI: 10.2210/pdb3ro5/pdb
• Descriptor: Nucleocapsid protein, [4-(2-chloro-4-nitrophenyl)piperazin-1-yl][3-(2-methoxyphenyl)-5-methyl-1,2-oxazol-4-yl]methanone (3 entities in total)
• Functional Keywords: influenza nucleoprotein, viral protein
• Biological source: Influenza A virus
• Total number of polymer chains: 2
• Total formula weight: 114525.85

Authors

Pearce, B.C.,Edavettal, S.,McDonnell, P.A.,Lewis, H.A.,Steinbacher, S.,Baldwin, E.T.,Langley, D.R.,Maskos, K.,Mortl, M.,Kiefersauer, R. (deposition date: 2011-04-25, release date: 2011-09-14, Last modification date: 2024-02-28)

Primary citation

Gerritz, S.W.,Cianci, C.,Kim, S.,Pearce, B.C.,Deminie, C.,Discotto, L.,McAuliffe, B.,Minassian, B.F.,Shi, S.,Zhu, S.,Zhai, W.,Pendri, A.,Li, G.,Poss, M.A.,Edavettal, S.,McDonnell, P.A.,Lewis, H.A.,Maskos, K.,Mortl, M.,Kiefersauer, R.,Steinbacher, S.,Baldwin, E.T.,Metzler, W.,Bryson, J.,Healy, M.D.,Philip, T.,Zoeckler, M.,Schartman, R.,Sinz, M.,Leyva-Grado, V.H.,Hoffmann, H.H.,Langley, D.R.,Meanwell, N.A.,Krystal, M.
Inhibition of influenza virus replication via small molecules that induce the formation of higher-order nucleoprotein oligomers.
Proc.Natl.Acad.Sci.USA, 108:15366-15371, 2011

PubMed Abstract: Influenza nucleoprotein (NP) plays multiple roles in the virus life cycle, including an essential function in viral replication as an integral component of the ribonucleoprotein complex, associating with viral RNA and polymerase within the viral core. The multifunctional nature of NP makes it an attractive target for antiviral intervention, and inhibitors targeting this protein have recently been reported. In a parallel effort, we discovered a structurally similar series of influenza replication inhibitors and show that they interfere with NP-dependent processes via formation of higher-order NP oligomers. Support for this unique mechanism is provided by site-directed mutagenesis studies, biophysical characterization of the oligomeric ligand:NP complex, and an X-ray cocrystal structure of an NP dimer of trimers (or hexamer) comprising three NP_A:NP_B dimeric subunits. Each NP_A:NP_B dimeric subunit contains two ligands that bridge two composite, protein-spanning binding sites in an antiparallel orientation to form a stable quaternary complex. Optimization of the initial screening hit produced an analog that protects mice from influenza-induced weight loss and mortality by reducing viral titers to undetectable levels throughout the course of treatment.

PubMed: 21896751
DOI: 10.1073/pnas.1107906108

Experimental method

X-RAY DIFFRACTION (2.66 Å)