3RNU

Structural Basis of Cytosolic DNA Sensing by Innate Immune Receptors

3RNU の概要

• エントリーDOI: 10.2210/pdb3rnu/pdb
• 関連するPDBエントリー: 3RLN 3RLO 3RN2 3RN5
• 分子名称: Gamma-interferon-inducible protein 16, DNA (5'-D(*GP*CP*CP*AP*TP*CP*AP*AP*AP*GP*AP*GP*AP*GP*AP*G)-3'), DNA (5'-D(*TP*CP*TP*CP*TP*CP*TP*TP*TP*GP*AP*TP*GP*GP*CP*C)-3'), ... (6 entities in total)
• 機能のキーワード: ob fold, dna binding, cytosolic dna sensor, cytosolic, immune response-dna complex, transcription activator-dna complex, transcription activator/dna
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus: Q16666
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 101735.74

構造登録者

Jin, T.C.,Xiao, T. (登録日: 2011-04-22, 公開日: 2012-04-25, 最終更新日: 2023-09-13)

主引用文献

Jin, T.,Perry, A.,Jiang, J.,Smith, P.,Curry, J.A.,Unterholzner, L.,Jiang, Z.,Horvath, G.,Rathinam, V.A.,Johnstone, R.W.,Hornung, V.,Latz, E.,Bowie, A.G.,Fitzgerald, K.A.,Xiao, T.S.
Structures of the HIN Domain:DNA Complexes Reveal Ligand Binding and Activation Mechanisms of the AIM2 Inflammasome and IFI16 Receptor.
Immunity, 36:561-571, 2012

PubMed Abstract: Recognition of DNA by the innate immune system is central to antiviral and antibacterial defenses, as well as an important contributor to autoimmune diseases involving self DNA. AIM2 (absent in melanoma 2) and IFI16 (interferon-inducible protein 16) have been identified as DNA receptors that induce inflammasome formation and interferon production, respectively. Here we present the crystal structures of their HIN domains in complex with double-stranded (ds) DNA. Non-sequence-specific DNA recognition is accomplished through electrostatic attraction between the positively charged HIN domain residues and the dsDNA sugar-phosphate backbone. An intramolecular complex of the AIM2 Pyrin and HIN domains in an autoinhibited state is liberated by DNA binding, which may facilitate the assembly of inflammasomes along the DNA staircase. These findings provide mechanistic insights into dsDNA as the activation trigger and oligomerization platform for the assembly of large innate signaling complexes such as the inflammasomes.

PubMed: 22483801
DOI: 10.1016/j.immuni.2012.02.014

実験手法

X-RAY DIFFRACTION (2.502 Å)