3RKQ

NKX2.5 Homeodomain dimer bound to ANF-242 DNA

3RKQ の概要

• エントリーDOI: 10.2210/pdb3rkq/pdb
• 分子名称: Homeobox protein Nkx-2.5, ANF-242 DNA, MAGNESIUM ION, ... (5 entities in total)
• 機能のキーワード: helix-turn-helix, dna binding, nucleus, transcription-dna complex, transcription/dna
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus (Probable): P52952
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 25934.38

構造登録者

Genis, C.,Scone, P.,Kasahara, H.,Nam, H.-J. (登録日: 2011-04-18, 公開日: 2012-05-09, 最終更新日: 2024-02-28)

主引用文献

Pradhan, L.,Genis, C.,Scone, P.,Weinberg, E.O.,Kasahara, H.,Nam, H.J.
Crystal structure of the human NKX2.5 homeodomain in complex with DNA target.
Biochemistry, 51:6312-6319, 2012

PubMed Abstract: NKX2.5 is a homeodomain containing transcription factor regulating cardiac formation and function, and its mutations are linked to congenital heart disease. Here we provide the first report of the crystal structure of the NKX2.5 homeodomain in complex with double-stranded DNA of its endogenous target, locating within the proximal promoter -242 site of the atrial natriuretic factor gene. The crystal structure, determined at 1.8 Å resolution, demonstrates that NKX2.5 homeodomains occupy both DNA binding sites separated by five nucleotides without physical interaction between themselves. The two homeodomains show identical conformation despite the differences in the DNA sequences they bind, and no significant bending of the DNA was observed. Tyr54, absolutely conserved in NK2 family proteins, mediates sequence-specific interaction with the TAAG motif. This high resolution crystal structure of NKX2.5 protein provides a detailed picture of protein and DNA interactions, which allows us to predict DNA binding of mutants identified in human patients.

PubMed: 22849347
DOI: 10.1021/bi300849c

実験手法

X-RAY DIFFRACTION (1.7 Å)