3RHY

Crystal structure of the dimethylarginine dimethylaminohydrolase adduct with 4-chloro-2-hydroxymethylpyridine

3RHY の概要

• エントリーDOI: 10.2210/pdb3rhy/pdb
• 関連するPDBエントリー: 1H70 3BPB
• 分子名称: N(G),N(G)-dimethylarginine dimethylaminohydrolase, (4-chloropyridin-2-yl)methanol (3 entities in total)
• 機能のキーワード: enzyme adduct, hydrolase
• 由来する生物種: Pseudomonas aeruginosa
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 57300.18

構造登録者

Monzingo, A.F.,Johnson, C.M.,Ke, Z.,Yoon, D.-W.,Linsky, T.W.,Guo, H.,Fast, W.,Robertus, J.D. (登録日: 2011-04-12, 公開日: 2011-06-15, 最終更新日: 2024-11-20)

主引用文献

Johnson, C.M.,Monzingo, A.F.,Ke, Z.,Yoon, D.W.,Linsky, T.W.,Guo, H.,Robertus, J.D.,Fast, W.
On the mechanism of dimethylarginine dimethylaminohydrolase inactivation by 4-halopyridines.
J.Am.Chem.Soc., 133:10951-10959, 2011

PubMed Abstract: Small molecules capable of selective covalent protein modification are of significant interest for the development of biological probes and therapeutics. We recently reported that 2-methyl-4-bromopyridine is a quiescent affinity label for the nitric oxide controlling enzyme dimethylarginine dimethylaminohydrolase (DDAH) (Johnson, C. M.; Linsky, T. W.; Yoon, D. W.; Person, M. D.; Fast, W. J. Am. Chem. Soc. 2011, 133, 1553-1562). Discovery of this novel protein modifier raised the possibility that the 4-halopyridine motif may be suitable for wider application. Therefore, the inactivation mechanism of the related compound 2-hydroxymethyl-4-chloropyridine is probed here in more detail. Solution studies support an inactivation mechanism in which the active site Asp66 residue stabilizes the pyridinium form of the inactivator, which has enhanced reactivity toward the active site Cys, resulting in covalent bond formation, loss of the halide, and irreversible inactivation. A 2.18 Å resolution X-ray crystal structure of the inactivated complex elucidates the orientation of the inactivator and its covalent attachment to the active site Cys, but the structural model does not show an interaction between the inactivator and Asp66. Molecular modeling is used to investigate inactivator binding, reaction, and also a final pyridinium deprotonation step that accounts for the apparent differences between the solution-based and structural studies with respect to the role of Asp66. This work integrates multiple approaches to elucidate the inactivation mechanism of a novel 4-halopyridine "warhead," emphasizing the strategy of using pyridinium formation as a "switch" to enhance reactivity when bound to the target protein.

PubMed: 21630706
DOI: 10.1021/ja2033684

実験手法

X-RAY DIFFRACTION (2.18 Å)