3RGV

A single TCR bound to MHCI and MHC II reveals switchable TCR conformers

3RGV の概要

• エントリーDOI: 10.2210/pdb3rgv/pdb
• 分子名称: Yae62 TCR a chain, Yae62 TCR b chain, H-2 class I histocompatibility antigen, K-B alpha chain, ... (5 entities in total)
• 機能のキーワード: tcr, mhc, mhc class i, immune system
• 由来する生物種: Mus musculus (mouse); 詳細
• 細胞内の位置: Membrane; Single-pass type I membrane protein: P01901; Secreted: P01887
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 93630.09

構造登録者

Dai, S.,Huseby, E.,Scott-Browne, J.,Rubtsova, K.,Pinilla, C.,Crawford, F.,Marrack, P.,Yin, L.,Kappler, J.W. (登録日: 2011-04-09, 公開日: 2011-07-20, 最終更新日: 2024-11-27)

主引用文献

Yin, L.,Huseby, E.,Scott-Browne, J.,Rubtsova, K.,Pinilla, C.,Crawford, F.,Marrack, P.,Dai, S.,Kappler, J.W.
A Single T Cell Receptor Bound to Major Histocompatibility Complex Class I and Class II Glycoproteins Reveals Switchable TCR Conformers.
Immunity, 35:23-33, 2011

PubMed Abstract: Major histocompatibility complex class I (MHCI) and MHCII proteins differ in structure and sequence. To understand how T cell receptors (TCRs) can use the same set of variable regions to bind both proteins, we have presented a comparison of a single TCR bound to both MHCI and MHCII ligands. The TCR adopts similar orientations on both ligands with TCR amino acids thought to be evolutionarily conserved for MHC interaction occupying similar positions on the MHCI and MHCII helices. However, the TCR antigen-binding loops use different conformations when interacting with each ligand. Most importantly, we observed alternate TCR core conformations. When bound to MHCI, but not MHCII, Vα disengages from the Jα β strand, switching Vα's position relative to Vβ. In several other structures, either Vα or Vβ undergoes this same modification. Thus, both TCR V-domains can switch among alternate conformations, perhaps extending their ability to react with different MHC-peptide ligands.

PubMed: 21683626
DOI: 10.1016/j.immuni.2011.04.017

実験手法

X-RAY DIFFRACTION (2.9 Å)