3RAN

CANINE GDP-RAN Q69L MUTANT

3RAN の概要

• エントリーDOI: 10.2210/pdb3ran/pdb
• 分子名称: PROTEIN (GTP-BINDING NUCLEAR PROTEIN RAN), MAGNESIUM ION, GUANOSINE-5'-DIPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: gtpase, nuclear transport, transport protein
• 由来する生物種: Canis lupus familiaris (dog)
• 細胞内の位置: Nucleus: P62825
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 99634.56

構造登録者

Stewart, M.,Kent, H.M.,Mccoy, A.J. (登録日: 1998-10-19, 公開日: 1998-12-02, 最終更新日: 2024-04-03)

主引用文献

Stewart, M.,Kent, H.M.,McCoy, A.J.
The structure of the Q69L mutant of GDP-Ran shows a major conformational change in the switch II loop that accounts for its failure to bind nuclear transport factor 2 (NTF2).
J.Mol.Biol., 284:1517-1527, 1998

PubMed Abstract: We report the 2.3 A resolution X-ray crystal structure of the GDP-bound form of the RanQ69L mutant that is used extensively in studies of nucleocytoplasmic transport and cell-cycle progression. When the structure of GDP-RanQ69L from monoclinic crystals with P21 symmetry was compared with the structure of wild-type Ran obtained from monoclinic crystals, the Q69L mutant showed a large conformational change in residues 68-74, which are in the switch II region of the molecule which changes conformation in response to nucleotide state and which forms the major interaction interface with nuclear transport factor 2 (NTF2, sometimes called p10). This conformational change alters the positions of key residues such as Lys71, Phe72 and Arg76 that are crucial for the interaction of GDP-Ran with NTF2 and indeed, solution binding studies were unable to detect any interaction between NTF2 and GDP-RanQ69L under conditions where GDP-Ran bound effectively. This interaction between NTF2 and GDP-Ran is required for efficient nuclear protein import and may function between the docking and translocation steps of the pathway.

PubMed: 9878368
DOI: 10.1006/jmbi.1998.2204

実験手法

X-RAY DIFFRACTION (2.15 Å)