3RAE

Quinolone(Levofloxacin)-DNA cleavage complex of type IV topoisomerase from S. pneumoniae

3RAE の概要

• エントリーDOI: 10.2210/pdb3rae/pdb
• 関連するPDBエントリー: 2NOV 3FOE 3FOF 3K9F 3KSA 3KSB 3LTN 3RAD 3RAF
• 分子名称: DNA topoisomerase 4 subunit A, DNA topoisomerase 4 subunit B, 5'-D(*CP*AP*TP*GP*AP*AP*T)-3', ... (9 entities in total)
• 機能のキーワード: protein-dna cleavage complex, topoisomerase iia, levofloxacin, isomerase-dna-antibiotic complex, isomerase/dna/antibiotic
• 由来する生物種: Streptococcus pneumoniae; 詳細
• 細胞内の位置: Cell membrane ; Peripheral membrane protein : P72525
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 185552.11

構造登録者

Laponogov, I.,Pan, X.-S.,Veselkov, D.A.,McAuley, K.E.,Fisher, L.M.,Sanderson, M.R. (登録日: 2011-03-28, 公開日: 2012-04-25, 最終更新日: 2024-10-30)

主引用文献

Veselkov, D.A.,Laponogov, I.,Pan, X.S.,Selvarajah, J.,Skamrova, G.B.,Branstrom, A.,Narasimhan, J.,Prasad, J.V.,Fisher, L.M.,Sanderson, M.R.
Structure of a quinolone-stabilized cleavage complex of topoisomerase IV from Klebsiella pneumoniae and comparison with a related Streptococcus pneumoniae complex.
Acta Crystallogr.,Sect.D, 72:488-496, 2016

PubMed Abstract: Klebsiella pneumoniae is a Gram-negative bacterium that is responsible for a range of common infections, including pulmonary pneumonia, bloodstream infections and meningitis. Certain strains of Klebsiella have become highly resistant to antibiotics. Despite the vast amount of research carried out on this class of bacteria, the molecular structure of its topoisomerase IV, a type II topoisomerase essential for catalysing chromosomal segregation, had remained unknown. In this paper, the structure of its DNA-cleavage complex is reported at 3.35 Å resolution. The complex is comprised of ParC breakage-reunion and ParE TOPRIM domains of K. pneumoniae topoisomerase IV with DNA stabilized by levofloxacin, a broad-spectrum fluoroquinolone antimicrobial agent. This complex is compared with a similar complex from Streptococcus pneumoniae, which has recently been solved.

PubMed: 27050128
DOI: 10.1107/S2059798316001212

実験手法

X-RAY DIFFRACTION (2.9 Å)